Electron microscopic analysis of heat-induced leakage of polyphosphate from a phoU mutant of Escherichia coli.

نویسندگان

  • Hitoshi Kashihara
  • Bong Mun Kang
  • Takeshi Omasa
  • Kohsuke Honda
  • Yuka Sameshima
  • Akio Kuroda
  • Hisao Ohtake
چکیده

Heat treatment is a simple technique for releasing polyphosphate (polyP) from microbial cells. We investigated the ultrastructural alterations of polyP granules (PPGs) in response to heat treatment at 70 degrees C using a polyP-accumulating phoU mutant of Escherichia coli as a model organism. Electron microscopic and energy-dispersive X-ray analysis suggested concurrent occurrence of PPG degradation and polyP release in the cytoplasm. PolyP is probably released by cytoplasmic extrusion through breaks in the cell envelope.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

PhoU enhances the ability of extraintestinal pathogenic Escherichia coli strain CFT073 to colonize the murine urinary tract.

The phoU gene is the last cistron in the pstSCAB-phoU operon and functions as a negative regulator of the Pho regulon. The authors previously identified a phoU mutant of extraintestinal pathogenic Escherichia coli strain CFT073 and demonstrated that this mutant was attenuated for survival in the murine model of ascending urinary tract infection. It is hypothesized that the PhoU protein might se...

متن کامل

Inorganic polyphosphate supports resistance and survival of stationary-phase Escherichia coli.

The Escherichia coli mutant (ppk) lacking the enzyme polyphosphate kinase, which makes long chains of inorganic polyphosphate (poly P), is deficient in functions expressed in the stationary phase of growth. After 2 days of growth in a medium limited in carbon sources, only 7% of the mutants survived compared with nearly 100% of the wild type; the loss in viability of the mutant was even more pr...

متن کامل

Two-Component Signal Transduction: a Special Issue in the Journal of Bacteriology.

Cover photograph: Transmission electron micrographs of wild-type (top) and phoU mutant (bottom) cells of the Gram-negative alphaproteobacterium Sinorhizobium meliloti. The cells were cultured in morpholinepropanesulfonic acid-buffered minimal medium with 7.5 mM glucose and 2 mM KH2PO4. Unlike the uniform rod-shaped wild-type cells, the phoU mutant cells are enlarged and pleomorphic, accumulate ...

متن کامل

Construction of an iss deleted mutant strain from a native avian pathogenic Escherichia coli O78: K80 and in vitro serum resistance evaluation of mutant

BACKGROUND: Colibacillosis, caused by different serotypes of avian pathogenic Escherichia coli (APEC), is one of the important diseases in poultry industry. The isolate O78 is the most prevalent serotype of APEC in Iran. One of the APEC virulence factors, increased serum survival (iss) gene, is related to serum resistance. The usual form of colibacillosis in avian is extraintestinal, and serum ...

متن کامل

Evaluation of antibacterial effect of magnesium oxide nanoparticles with nisin and heat in milk

Objective(s): The objective of this study was to investigate the antibacterial activities of magnesium oxide nanoparticles (MgO NP) alone or in combination with other antimicrobials (nisin and heat) against Escherichia coli and Staphylococcus aureus in milk.Materials and Methods: First, the combined effect of nisin and MgO nanoparticles was investigated in milk. Then the combined effect of nisi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Bioscience, biotechnology, and biochemistry

دوره 74 4  شماره 

صفحات  -

تاریخ انتشار 2010